Ready to zap some cells 🔌 ⠀⠀⠀⠀⠀⠀⠀⠀⠀
One of the techniques I use frequently in the lab is called electroporation. To do this, I place cells suspended in a small volume of liquid into the bottom of a little blue cuvette. The cuvette is then placed into a machine that creates a small electrical field that passes through the cells. Essentially this electrical field is punching loads of tiny holes into the cell to increase the permeability of the cell membrane. For scientists 🥼 this means we now have a way to introduce different biological products.
For me, I use this technique to introduce small interfering RNA 🧬 into the cell, which can silence (turn off) specific genes! Although the technique is quite harsh, the cells tend to recover well 💪🏻. ⠀⠀⠀⠀⠀⠀⠀⠀⠀
The main downsides to this technique is that effects are only transient, meaning the silencing only lasts for a couple of days.
Look at these huge cell pellets! I’ve been struggling with cell growth lately, so I was so happy to see the large amount of cells I was able to collect! This week I also learned real time PCR, which turns out is only slightly more glamorous than a regular PCR in terms of set up, but the data is so so cool! I’m excited to graduate in just three weeks and then I can be in the lab full time! #undergradresearch#gradschool#science#microbiology#immunology#lablife#research#cellbiology
Yesterday was my baby's 9th birthday!!! We didnt have time to make his pupcakes so we celebrated today!! He got his pupcakes, ball and huge stuffed doughnut he ripped apart already hahaha happy birthday old man!!!! These are totally safe to eat. They're actually made for dogs. It's not human cake!!!!! #yellowlab#labsofinstagram#gunnythelab#lablife#furbaby
Tomorrow you‘ll find me right there in this spot. With my lighthouse toy. I would tell you how excited I am but we just came back from a 5km walk and now I‘m pretty tired. I‘m not used to those temperatures anymore. My humans ordered 🍣 and - as they always do - ordered way too much. So I‘ll look at them with my „starving“ eyes and will get my fair share. I mean - if it wasn‘t for me they wouldn‘t get their butts up and go for walks to earn those sushi calories. Don‘t you think I have a point there? How about you guys? Do your humans share their food with you or should I start an online course „How to manipulate your humans with just one glance“ for all of you? Let me know what you think! #lab#lablife#labrador#labradorretriever#labsofinsta#labradorsofinstagram#silverlab#silverlabrador#labproblems#silverlaboftheday#silverlab_squad#silverlabsquad#laboftheday
Happy Friday! 😂
I just thought we'd have some fun today with this picture 🤷 what causes you to make this face? 🙈
I'll start: My face when I see a new argument floating around the anti-vax community 😂
. #thecurioussheep#science#scicomm#tgif#myfacewhen #💙