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lablife - 635.9k posts

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  • Ready to zap some cells 🔌 ⠀⠀⠀⠀⠀⠀⠀⠀⠀
One of the techniques I use frequently in the lab is called electroporation. To do this, I place cells suspended in a small volume of liquid into the bottom of a little blue cuvette. The cuvette is then placed into a machine that creates a small electrical field that passes through the cells. Essentially this electrical field is punching loads of tiny holes into the cell to increase the permeability of the cell membrane. For scientists 🥼 this means we now have a way to introduce different biological products.
⠀⠀⠀⠀⠀⠀⠀⠀⠀
For me, I use this technique to introduce small interfering RNA 🧬 into the cell, which can silence (turn off) specific genes! Although the technique is quite harsh, the cells tend to recover well 💪🏻. ⠀⠀⠀⠀⠀⠀⠀⠀⠀
The main downsides to this technique is that effects are only transient, meaning the silencing only lasts for a couple of days.
  • Ready to zap some cells 🔌 ⠀⠀⠀⠀⠀⠀⠀⠀⠀
    One of the techniques I use frequently in the lab is called electroporation. To do this, I place cells suspended in a small volume of liquid into the bottom of a little blue cuvette. The cuvette is then placed into a machine that creates a small electrical field that passes through the cells. Essentially this electrical field is punching loads of tiny holes into the cell to increase the permeability of the cell membrane. For scientists 🥼 this means we now have a way to introduce different biological products.
    ⠀⠀⠀⠀⠀⠀⠀⠀⠀
    For me, I use this technique to introduce small interfering RNA 🧬 into the cell, which can silence (turn off) specific genes! Although the technique is quite harsh, the cells tend to recover well 💪🏻. ⠀⠀⠀⠀⠀⠀⠀⠀⠀
    The main downsides to this technique is that effects are only transient, meaning the silencing only lasts for a couple of days.
  • 888 43 16 April, 2019

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  • Henry, doing his best “feel sorry for me” face. #dramaking
  • Henry, doing his best “feel sorry for me” face. #dramaking
  • 144 4 44 minutes ago
  • Waiting for that guy carrying his pizza to drop me a slice @sizzlepie
  • Waiting for that guy carrying his pizza to drop me a slice @sizzlepie
  • 1,707 27 15 April, 2019

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  • Look at these huge cell pellets! I’ve been struggling with cell growth lately, so I was so happy to see the large amount of cells I was able to collect! This week I also learned real time PCR, which turns out is only slightly more glamorous than a regular PCR in terms of set up, but the data is so so cool! I’m excited to graduate in just three weeks and then I can be in the lab full time! #undergradresearch #gradschool #science #microbiology #immunology #lablife #research #cellbiology
  • Look at these huge cell pellets! I’ve been struggling with cell growth lately, so I was so happy to see the large amount of cells I was able to collect! This week I also learned real time PCR, which turns out is only slightly more glamorous than a regular PCR in terms of set up, but the data is so so cool! I’m excited to graduate in just three weeks and then I can be in the lab full time! #undergradresearch #gradschool #science #microbiology #immunology #lablife #research #cellbiology
  • 987 12 14 April, 2019

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  • Henry, doing his best “feel sorry for me” face. #dramaking
  • Henry, doing his best “feel sorry for me” face. #dramaking
  • 144 4 44 minutes ago

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  • Yesterday was my baby's 9th birthday!!! We didnt have time to make his pupcakes so we celebrated today!! He got his pupcakes, ball and huge stuffed doughnut he ripped apart already hahaha happy birthday old man!!!! These are totally safe to eat. They're actually made for dogs. It's not human cake!!!!! #yellowlab #labsofinstagram #gunnythelab #lablife #furbaby
  • Yesterday was my baby's 9th birthday!!! We didnt have time to make his pupcakes so we celebrated today!! He got his pupcakes, ball and huge stuffed doughnut he ripped apart already hahaha happy birthday old man!!!! These are totally safe to eat. They're actually made for dogs. It's not human cake!!!!! #yellowlab #labsofinstagram #gunnythelab #lablife #furbaby
  • 6 2 58 minutes ago

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  • Den Tag schließen wir mit einem Ausflug zur Elbe ab. 🐕 Jetzt bin ich fertig und müde 💤
  • Den Tag schließen wir mit einem Ausflug zur Elbe ab. 🐕 Jetzt bin ich fertig und müde 💤
  • 39 8 1 hour ago
  • Had the BEST holiday running around with my best furiend. Then spent this evening chilling in the sun watching all the 🐑 😃🐶
  • Had the BEST holiday running around with my best furiend. Then spent this evening chilling in the sun watching all the 🐑 😃🐶
  • 10 1 1 hour ago
  • Wir genießen unser Wochenende schon 😍 ihr auch?

Amy hat zur Zeit mal wieder eine Phase in der man ihr alles 3 mal sagen muss 🙄 ganz schön nervig. Ist das bei euch auch manchmal so?

Morgen geht es an den Hundestrand 😍😊 das Wetter ist ja perfekt dafür.
  • Wir genießen unser Wochenende schon 😍 ihr auch?

    Amy hat zur Zeit mal wieder eine Phase in der man ihr alles 3 mal sagen muss 🙄 ganz schön nervig. Ist das bei euch auch manchmal so?

    Morgen geht es an den Hundestrand 😍😊 das Wetter ist ja perfekt dafür.
  • 29 1 1 hour ago
  • Running into this weekend like
  • Running into this weekend like
  • 27 2 1 hour ago
  • 🎂
  • 🎂
  • 13 2 1 hour ago
  • Outfit primaverile👩🏻‍🔬😷🌸 #lablife
  • Outfit primaverile👩🏻‍🔬😷🌸 #lablife
  • 59 3 1 hour ago
  • Just call me tigger
  • Just call me tigger
  • 13 3 1 hour ago
  • Princess Ruby👑
  • Princess Ruby👑
  • 12 1 1 hour ago
  • 🌟
  • 🌟
  • 14 1 1 hour ago
  • Tomorrow you‘ll find me right there in this spot. With my lighthouse toy. I would tell you how excited I am but we just came back from a 5km walk and now I‘m pretty tired. I‘m not used to those temperatures anymore. My humans ordered 🍣 and - as they always do - ordered way too much. So I‘ll look at them with my „starving“ eyes and will get my fair share. I mean - if it wasn‘t for me they wouldn‘t get their butts up and go for walks to earn those sushi calories. Don‘t you think I have a point there? How about you guys? Do your humans share their food with you or should I start an online course „How to manipulate your humans with just one glance“ for all of you? Let me know what you think! #lab #lablife #labrador #labradorretriever #labsofinsta #labradorsofinstagram #silverlab #silverlabrador #labproblems #silverlaboftheday #silverlab_squad #silverlabsquad #laboftheday
  • Tomorrow you‘ll find me right there in this spot. With my lighthouse toy. I would tell you how excited I am but we just came back from a 5km walk and now I‘m pretty tired. I‘m not used to those temperatures anymore. My humans ordered 🍣 and - as they always do - ordered way too much. So I‘ll look at them with my „starving“ eyes and will get my fair share. I mean - if it wasn‘t for me they wouldn‘t get their butts up and go for walks to earn those sushi calories. Don‘t you think I have a point there? How about you guys? Do your humans share their food with you or should I start an online course „How to manipulate your humans with just one glance“ for all of you? Let me know what you think! #lab #lablife #labrador #labradorretriever #labsofinsta #labradorsofinstagram #silverlab #silverlabrador #labproblems #silverlaboftheday #silverlab_squad #silverlabsquad #laboftheday
  • 21 0 1 hour ago
  • Happy Friday! 😂
.
I just thought we'd have some fun today with this picture 🤷 what causes you to make this face? 🙈
.
I'll start: My face when I see a new argument floating around the anti-vax community 😂
.
.
.
#thecurioussheep #science #scicomm #tgif #myfacewhen #💙
  • Happy Friday! 😂
    .
    I just thought we'd have some fun today with this picture 🤷 what causes you to make this face? 🙈
    .
    I'll start: My face when I see a new argument floating around the anti-vax community 😂
    .
    .
    .
    #thecurioussheep #science #scicomm #tgif #myfacewhen #💙
  • 79 30 1 hour ago
  • Labrainbow!🌈🐾 If you’re looking for the next perfect member of your family head to @zonalabradors for some available puppies! Especially if you love 🍫!
  • Labrainbow!🌈🐾 If you’re looking for the next perfect member of your family head to @zonalabradors for some available puppies! Especially if you love 🍫!
  • 262 5 1 hour ago
  • That crushed ice tap water hash ......... Trying to spread truth out here in a sea of haters.....
  • That crushed ice tap water hash ......... Trying to spread truth out here in a sea of haters.....
  • 148 15 2 hours ago
  • Well folks de springtime haz reely sprung today!!! I is absolutee luvin soakin up de rays in me thick golden locks. All i can say is... BRING ON DE SUMMER!!! Like if ya feelin gud on dis glorious gud Friday😎😜🥰☀️🌳🐇#lablife #labsofinstagram #goodfriday #modellife #goldenhour 💚💛
  • Well folks de springtime haz reely sprung today!!! I is absolutee luvin soakin up de rays in me thick golden locks. All i can say is... BRING ON DE SUMMER!!! Like if ya feelin gud on dis glorious gud Friday😎😜🥰☀️🌳🐇 #lablife #labsofinstagram #goodfriday #modellife #goldenhour 💚💛
  • 19 2 2 hours ago
  • That's crushed ice and water tech 🤣😂🤣🤣😂🤣😂🤣 that was a good reddit post yesterday. Seriously tho. Find you some of these and try them for for yourself before "assuming" ...............whatever.
  • That's crushed ice and water tech 🤣😂🤣🤣😂🤣😂🤣 that was a good reddit post yesterday. Seriously tho. Find you some of these and try them for for yourself before "assuming" ...............whatever.
  • 143 22 2 hours ago
  • TGIF!😁
  • TGIF!😁
  • 21 2 2 hours ago
  • Happy its acupuncture day!
  • Happy its acupuncture day!
  • 256 7 2 hours ago